Send to:

Choose Destination
See comment in PubMed Commons below
Talanta. 2001 May 30;54(4):681-6.

Effects of fungal laccase immobilization procedures for the development of a biosensor for phenol compounds.

Author information

  • 1Instituto de Química, Universidade Estadual de Campinas, CP 6154, CEP 13083-970, Campinas, SP, Brazil.


Fungal laccase was immobilized on carbon-fiber electrodes using classical methods: physical adsorption, glutaraldehyde, carbodiimide and carbodiimide/glutaraldehyde. The highest biosensor response was obtained using carbodiimide/glutaraldehyde for coupling laccase to carboxyl groups on the carbon fibers. In this method, different percentages of glutaraldehyde had important effects on the sensitivity of the biosensor, the best percentage of glutaraldehyde being 10% (m/v). The behavior of the obtained biosensor was investigated in terms of sensitivity, operational range, pH and applied potential. The developed biosensor showed an optimum response at pH 5.0 and at an applied potential of -100 mV. The immobilized laccase retained a good activity for over 2 months.

PubMed Commons home

PubMed Commons

How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Write to the Help Desk