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Talanta. 1994 Mar;41(3):401-6.

Improvement of latex piezoelectric immunoassay: Detection of rheumatoid factor.

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  • 1Department of Biophysical Chemistry, Faculty of Pharmaceutical Sciences, Hokkaido University, North 12, West 6, Kita-ku, Sapporo 060, Japan.


Latex piezoelectric immunoassay is a method for detection of agglutination of antibody- or antigen-bearing latex by immunoreaction using a piezoelectric quartz crystal; the agglutination decreases the oscillation frequency of the crystal. This is advantageous in that coating the surface of the crystal followed by fixation of antibody or antigen is unnecessary. There is, however, a drawback, and to improve this, we designed a micro-cell in which only one side of the crystal is exposed to the solution. A method for regenerating the crystal was also devised. Measurement was carried out using a calibration curve of the frequency change against rheumatoid factor activity. The improvement made it possible to use one crystal repeatedly and reproducibility was satisfactory. The calibration curve became almost independent of the crystal used.

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