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    Nat Methods. 2008 Nov;5(11):955-7. Epub 2008 Oct 26.

    A noncytotoxic DsRed variant for whole-cell labeling.

    Source

    Department of Biochemistry and Molecular Biology, The University of Chicago, Gordon Center W238, Chicago, Illinois 60637, USA.

    Abstract

    A common application of fluorescent proteins is to label whole cells, but many RFPs are cytotoxic when used with standard high-level expression systems. We engineered a rapidly maturing tetrameric fluorescent protein called DsRed-Express2 that has minimal cytotoxicity. DsRed-Express2 exhibits strong and stable expression in bacterial and mammalian cells, and it outperforms other available RFPs with regard to photostability and phototoxicity.

    PMID:
    18953349
    [PubMed - indexed for MEDLINE]

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