Rfam: updates to the RNA families database.
Gardner PP,
Daub J,
Tate JG,
Nawrocki EP,
Kolbe DL,
Lindgreen S,
Wilkinson AC,
Finn RD,
Griffiths-Jones S,
Eddy SR,
Bateman A.
Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, CB10 1SA, UK. pg5@sanger.ac.uk
Rfam is a collection of RNA sequence families, represented by multiple sequence alignments and covariance models (CMs). The primary aim of Rfam is to annotate new members of known RNA families on nucleotide sequences, particularly complete genomes, using sensitive BLAST filters in combination with CMs. A minority of families with a very broad taxonomic range (e.g. tRNA and rRNA) provide the majority of the sequence annotations, whilst the majority of Rfam families (e.g. snoRNAs and miRNAs) have a limited taxonomic range and provide a limited number of annotations. Recent improvements to the website, methodologies and data used by Rfam are discussed. Rfam is freely available on the Web at http://rfam.sanger.ac.uk/and http://rfam.janelia.org/.
PMID: 18953034 [PubMed - indexed for MEDLINE]
PMCID: PMC2686503