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Anal Bioanal Chem. 2008 Dec;392(7-8):1291-7. doi: 10.1007/s00216-008-2433-x. Epub 2008 Oct 24.

Prospects for monolithic nano-LC columns in shotgun proteomics.

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  • 1CEA, iRTSV, Laboratoire d'Etude de la Dynamique des Protéomes, Grenoble, 38054, France. vilem.guryca@gmail.com

Abstract

We report a premier side-by-side comparison of two leading types of monolithic nano-LC column (silica-C(18), polystyrene) in shotgun proteomics experiments. Besides comparing the columns in terms of the number of peptides from a real-life sample (Arabidopsis thaliana chloroplast) that they identified, we compared the monoliths in terms of peak capacity and retention behavior for standard peptides. For proteomics applications where the mobile phase composition is constrained by electrospray ionization considerations (i.e., there is a restricted choice of ion-pairing modifiers), the polystyrene nano-LC column exhibited reduced identification power. The silica monolith column was superior in all measured values and compared very favorably with traditional packed columns. Finally, we investigated the performances of the monoliths at high flow rates in an attempt to demonstrate their advantages for high-throughput identification.

PMID:
18949466
[PubMed - indexed for MEDLINE]
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