J Infect Dis. 1991 Oct;164(4):773-6.

A method for the quantification of intracellular zidovudine nucleotides.

Kuster H, Vogt M, Joos B, Nadai V, Lüthy R.

Source

Department of Medicine, University Hospital, Zurich, Switzerland.

Abstract

An assay to quantify the phosphorylation products of zidovudine (AZT) in peripheral blood mononuclear cells (PBMC) was developed. Extracts of PBMC were separated by high-performance liquid chromatography. Eluted AZT mono- (MP), di- (DP), and triphosphate (TP) were collected in separate portions. Treatment with alkaline phosphatase yielded equimolar amounts of AZT, which after solid-phase enrichment were assayed by radioimmunoassay. Detection limit was 0.1 pmol/10(6) PBMC for each nucleotide. Recoveries of 102%-118% were observed. AZT nucleotides were measured in samples from three patients receiving 250 mg of AZT every 12 h. Intracellular concentrations of AZT-MP after 1-2 h ranged from 0.9 to 1.4 pmol/10(6) PBMC and then declined to 0.3-1.1 pmol/10(6) PBMC after 4 h. AZT-DP and AZT-TP reached concentrations of 0.3-0.5 pmol/10(6) PBMC after 1-2 h and could not be detected after 4 h in any of the three patients. Duplicate determinations deviated by less than 20%.

PMID:: 1894938; [PubMed - indexed for MEDLINE]

LinkOut - more resources

Full Text Sources

Molecular Biology Databases

ZIDOVUDINE - HSDB

Supplemental Content

Save items

Related citations in PubMed

A sensitive nonisotopic method for the determination of intracellular azidothymidine 5'-mono-, 5'-di-, and 5'-triphosphate. [Anal Biochem. 1991]
A sensitive nonisotopic method for the determination of intracellular azidothymidine 5'-mono-, 5'-di-, and 5'-triphosphate.
Toyoshima T, Kimura S, Muramatsu S, Takahagi H, Shimada K. Anal Biochem. 1991 Aug 1; 196(2):302-7.
Improved method to quantify intracellular zidovudine mono- and triphosphate in peripheral blood mononuclear cells by liquid chromatography-tandem mass spectrometry. [J Mass Spectrom. 2007]
Improved method to quantify intracellular zidovudine mono- and triphosphate in peripheral blood mononuclear cells by liquid chromatography-tandem mass spectrometry.
Compain S, Durand-Gasselin L, Grassi J, Benech H. J Mass Spectrom. 2007 Mar; 42(3):389-404.
Intracellular zidovudine (ZDV) and ZDV phosphates as measured by a validated combined high-pressure liquid chromatography-radioimmunoassay procedure. [Antimicrob Agents Chemother. 1...]
Intracellular zidovudine (ZDV) and ZDV phosphates as measured by a validated combined high-pressure liquid chromatography-radioimmunoassay procedure.
Slusher JT, Kuwahara SK, Hamzeh FM, Lewis LD, Kornhauser DM, Lietman PS. Antimicrob Agents Chemother. 1992 Nov; 36(11):2473-7.
Development of a direct assay for measuring intracellular AZT triphosphate in humans peripheral blood mononuclear cells. [Anal Chem. 2002]
Development of a direct assay for measuring intracellular AZT triphosphate in humans peripheral blood mononuclear cells.
Becher F, Schlemmer D, Pruvost A, Nevers MC, Goujard C, Jorajuria S, Guerreiro C, Brossette T, Lebeau L, Créminon C, et al. Anal Chem. 2002 Aug 15; 74(16):4220-7.
Review Mitochondria as cell targets of AZT (zidovudine). [Gen Pharmacol. 1998]
Review Mitochondria as cell targets of AZT (zidovudine).
Barile M, Valenti D, Quagliariello E, Passarella S. Gen Pharmacol. 1998 Oct; 31(4):531-8.

See reviews... See all...

Cited by 11 PubMed Central articles

Lack of evidence for in vivo transformation of zidovudine triphosphate to stavudine triphosphate in human immunodeficiency virus-infected patients. [Antimicrob Agents Chemother. 2...]
Lack of evidence for in vivo transformation of zidovudine triphosphate to stavudine triphosphate in human immunodeficiency virus-infected patients.
Meléndez M, Blanco R, Delgado W, García R, Santana J, García H, Rosario O, Rodríguez JF. Antimicrob Agents Chemother. 2006 Mar; 50(3):835-40.
Simultaneous quantitation of intracellular zidovudine and lamivudine triphosphates in human immunodeficiency virus-infected individuals. [Antimicrob Agents Chemother. 2...]
Simultaneous quantitation of intracellular zidovudine and lamivudine triphosphates in human immunodeficiency virus-infected individuals.
Rodriguez JF, Rodriguez JL, Santana J, García H, Rosario O. Antimicrob Agents Chemother. 2000 Nov; 44(11):3097-100.
Determination of zidovudine triphosphate intracellular concentrations in peripheral blood mononuclear cells from human immunodeficiency virus-infected individuals by tandem mass spectrometry. [Antimicrob Agents Chemother. 1...]
Determination of zidovudine triphosphate intracellular concentrations in peripheral blood mononuclear cells from human immunodeficiency virus-infected individuals by tandem mass spectrometry.
Font E, Rosario O, Santana J, García H, Sommadossi JP, Rodriguez JF. Antimicrob Agents Chemother. 1999 Dec; 43(12):2964-8.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Compound (MeSH Keyword)
PubChem chemical compound records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

A method for the quantification of intracellular zidovudine nucleotides.
A method for the quantification of intracellular zidovudine nucleotides.
J Infect Dis. 1991 Oct ;164(4):773-6.

PubMed
Co-targeting the EGFR and IGF-IR with anti-EGFR monoclonal antibody ICR62 and th...
Co-targeting the EGFR and IGF-IR with anti-EGFR monoclonal antibody ICR62 and the IGF-IR tyrosine kinase inhibitor NVP-AEW541 in colorectal cancer cells.
Int J Oncol. 2008 Nov ;33(5):1107-13.

PubMed
Epidemics and agendas: the politics of nightly news coverage of AIDS.
Epidemics and agendas: the politics of nightly news coverage of AIDS.
J Health Polit Policy Law. 1991 Summer ;16(2):215-49.

PubMed
An estimate of the global prevalence and incidence of herpes simplex virus type ...
An estimate of the global prevalence and incidence of herpes simplex virus type 2 infection.
Bull World Health Organ. 2008 Oct ;86(10):805-12, A.

PubMed
Treatment of mental disorders for adolescents in Mexico City.
Treatment of mental disorders for adolescents in Mexico City.
Bull World Health Organ. 2008 Oct ;86(10):757-64.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to:

A method for the quantification of intracellular zidovudine nucleotides.

Source

Abstract

Publication Types, MeSH Terms, Substances

Publication Types

MeSH Terms

Substances

LinkOut - more resources

Full Text Sources

Molecular Biology Databases

Supplemental Content

Save items

Related citations in PubMed

Cited by 11 PubMed Central articles

Related information

Recent activity

Simple NCBI Directory

Getting Started

Resources

Popular

Featured

NCBI Information