Display Settings:

Format

Send to:

Choose Destination
See comment in PubMed Commons below
BMC Bioinformatics. 2008 Oct 23;9:453. doi: 10.1186/1471-2105-9-453.

Probe signal correction for differential methylation hybridization experiments.

Author information

  • 1Human Cancer Genetics Program, OSU Comprehensive Cancer Center, The Ohio State University, Columbus, OH, USA. potterdp@gmail.com

Abstract

BACKGROUND:

Non-biological signal (or noise) has been the bane of microarray analysis. Hybridization effects related to probe-sequence composition and DNA dye-probe interactions have been observed in differential methylation hybridization (DMH) microarray experiments as well as other effects inherent to the DMH protocol.

RESULTS:

We suggest two models to correct for non-biologically relevant probe signal with an overarching focus on probe-sequence composition. The estimated effects are evaluated and the strengths of the models are considered in the context of DMH analyses.

CONCLUSION:

The majority of estimated parameters were statistically significant in all considered models. Model selection for signal correction is based on interpretation of the estimated values and their biological significance.

PMID:
18947421
[PubMed - indexed for MEDLINE]
PMCID:
PMC2603337
Free PMC Article

Images from this publication.See all images (6)Free text

Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for BioMed Central Icon for PubMed Central
    Loading ...
    Write to the Help Desk