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PLoS One. 2008;3(10):e3504. doi: 10.1371/journal.pone.0003504. Epub 2008 Oct 23.

Dynein-dynactin complex is essential for dendritic restriction of TM1-containing Drosophila Dscam.

Author information

  • 1Department of Neurobiology, University of Massachusetts Medical School, Worcester, MA, USA.

Abstract

BACKGROUND:

Many membrane proteins, including Drosophila Dscam, are enriched in dendrites or axons within neurons. However, little is known about how the differential distribution is established and maintained.

METHODOLOGY/PRINCIPAL FINDINGS:

Here we investigated the mechanisms underlying the dendritic targeting of Dscam[TM1]. Through forward genetic mosaic screens and by silencing specific genes via targeted RNAi, we found that several genes, encoding various components of the dynein-dynactin complex, are required for restricting Dscam[TM1] to the mushroom body dendrites. In contrast, compromising dynein/dynactin function did not affect dendritic targeting of two other dendritic markers, Nod and Rdl. Tracing newly synthesized Dscam[TM1] further revealed that compromising dynein/dynactin function did not affect the initial dendritic targeting of Dscam[TM1], but disrupted the maintenance of its restriction to dendrites.

CONCLUSIONS/SIGNIFICANCE:

The results of this study suggest multiple mechanisms of dendritic protein targeting. Notably, dynein-dynactin plays a role in excluding dendritic Dscam, but not Rdl, from axons by retrograde transport.

PMID:
18946501
[PubMed - indexed for MEDLINE]
PMCID:
PMC2566808
Free PMC Article

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