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    Cancer Chemother Pharmacol. 2009 Jun;64(1):79-86. doi: 10.1007/s00280-008-0845-0. Epub 2008 Oct 22.

    Evaluation of mRNA by Q-RTPCR and protein expression by AQUA of the M2 subunit of ribonucleotide reductase (RRM2) in human tumors.

    Kolesar J, Huang W, Eickhoff J, Hahn K, Alberti D, Attia S, Schelman W, Holen K, Traynor A, Ivy P, Wilding G.

    Source

    University of Wisconsin Paul P Carbone Comprehensive Cancer Center, Madison, WI 53792, USA. jmkolesar@pharmacy.wisc.edu

    Abstract

    PURPOSE:

    The purpose of this study was to evaluate baseline RRM2 protein and gene expression in tumors of patients receiving 3-AP.

    METHODS:

    Tumor blocks from patients enrolled in phase I and II clinical studies using 3-AP, were evaluated for RRM2 gene and protein expression by quantitative real time polymerase chain reaction (Q-RTPCR) and automated quantitative analysis (AQUA).

    RESULTS:

    Esophageal and gastric cancers overexpressed RRM2 protein when compared to prostate cancer (Z-score, 0.68 +/- 0.94 SD, vs 0.41 +/- 0.84 SD, respectively; p = 0.04). Esophageal and gastric cancers also overexpressed RRM2 mRNA when compared to prostate cancer (relative gene expression 2.56 +/- 1.49 SD, vs 0.29 +/- 0.20 SD, respectively; p = 0.02). Protein and gene expression were moderately associated (Spearman's rank correlation = 0.30; p = 0.12).

    CONCLUSION:

    RRM2 gene and protein expression varies by tumor type.

    PMID:
    18941749
    [PubMed - indexed for MEDLINE]
    PMCID:
    PMC3043989
    Free PMC Article

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