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PLoS One. 2008;3(10):e3457. doi: 10.1371/journal.pone.0003457. Epub 2008 Oct 20.

A high-throughput assay for siRNA-based circadian screens in human U2OS cells.

Author information

  • 1Regulatory Biology Laboratory, Salk Institute for Biological Studies, La Jolla, California, United States of America.

Erratum in

  • PLoS ONE. 2009;4(2).doi: 10.1371/annotation/42c80798-20f2-4a1f-8c1d-290631231cf5..

Abstract

The advent of siRNA-based screens has revolutionized the efficiency by which functional components of biological processes are identified. A notable exception has been the field of mammalian circadian rhythms. Here, we outline a medium- to high-throughput siRNA-based approach that, in combination with real-time bioluminescence measurement of a circadian reporter gene, can be utilized to elucidate the effects of gene knockdown across several days in human cells.

PMID:
18941634
[PubMed - indexed for MEDLINE]
PMCID:
PMC2565498
Free PMC Article
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