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J Clin Microbiol. 2008 Dec;46(12):4091-4. doi: 10.1128/JCM.01349-08. Epub 2008 Oct 15.

Combined multilocus short-sequence-repeat and mycobacterial interspersed repetitive unit-variable-number tandem-repeat typing of Mycobacterium avium subsp. paratuberculosis isolates.

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  • 1INRA, UR1282, Infectiologie Animale, Santé Publique, Nouzilly, France.

Abstract

Short-sequence-repeat (SSR) sequencing was applied to 127 Mycobacterium avium subsp. paratuberculosis isolates typed by mycobacterial interspersed repetitive unit-variable-number tandem repeats (MIRU-VNTR) and IS900 restriction fragment length polymorphism (RFLP). Combined MIRU-VNTR and SSR typing followed by secondary IS900 RFLP typing is an improved approach to high-resolution genotyping of this pathogen.

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