Biochem Soc Trans. 1991 Apr;19(2):432-7.

Fluorescence approaches to the study of the p21ras GTPase mechanism.

Eccleston JF, Moore KJ, Brownbridge GG, Webb MR, Lowe PN.

Source

Division of Physical Biochemistry, National Institute for Medical Research, Mill Hill, London, U.K.

Abstract

The use of ribose-modified guanine nucleotides and tryptophan mutants of p21ras, neither of which have significant effect on the kinetic mechanism of the p21ras GTPase and the GAP-activated p21ras GTPase, will now allow a detailed kinetic study of how GAP and other regulatory proteins interact with p21ras. This will lead to a better understanding of how the relative concentrations of 'active' p21ras. GTP and 'inactive' p21ras. GDP are regulated in the cell.

PMID:: 1889625; [PubMed - indexed for MEDLINE]

Publication Types, MeSH Terms, Substances

Publication Types

MeSH Terms

Substances

LinkOut - more resources

Full Text Sources

Portland Press - PDF

Supplemental Content

Save items

Related citations in PubMed

Interaction of GTPase activating proteins (GAPs) with p21ras measured by a novel fluorescence anisotropy method. Essential role of Arg-903 of GAP in activation of GTP hydrolysis on p21ras. [J Biol Chem. 1993]
Interaction of GTPase activating proteins (GAPs) with p21ras measured by a novel fluorescence anisotropy method. Essential role of Arg-903 of GAP in activation of GTP hydrolysis on p21ras.
Brownbridge GG, Lowe PN, Moore KJ, Skinner RH, Webb MR. J Biol Chem. 1993 May 25; 268(15):10914-9.
Linear free energy relationships in the intrinsic and GTPase activating protein-stimulated guanosine 5'-triphosphate hydrolysis of p21ras. [Biochemistry. 1996]
Linear free energy relationships in the intrinsic and GTPase activating protein-stimulated guanosine 5'-triphosphate hydrolysis of p21ras.
Schweins T, Geyer M, Kalbitzer HR, Wittinghofer A, Warshel A. Biochemistry. 1996 Nov 12; 35(45):14225-31.
A synthetic peptide corresponding to a sequence in the GTPase activating protein inhibits p21ras stimulation and promotes guanine nucleotide exchange. [Int J Pept Protein Res. 1991]
A synthetic peptide corresponding to a sequence in the GTPase activating protein inhibits p21ras stimulation and promotes guanine nucleotide exchange.
Rubinfeld B, Wong G, Bekesi E, Wood A, Heimer E, McCormick F, Polakis P. Int J Pept Protein Res. 1991 Jul; 38(1):47-53.
Review The kinetic mechanism of the GAP-activated GTPase of p21 ras. [Philos Trans R Soc Lond B Biol...]
Review The kinetic mechanism of the GAP-activated GTPase of p21 ras.
Moore KJ, Lowe PN, Eccleston JF. Philos Trans R Soc Lond B Biol Sci. 1992 Apr 29; 336(1276):49-53; discussion 53-4.
Review The effector interactions of p21ras. [Trends Biochem Sci. 1993]
Review The effector interactions of p21ras.
Marshall MS. Trends Biochem Sci. 1993 Jul; 18(7):250-4.

See reviews... See all...

Cited by 5 PubMed Central articles

Spontaneous nucleotide exchange in low molecular weight GTPases by fluorescently labeled gamma-phosphate-linked GTP analogs. [Proc Natl Acad Sci U S A. 2004]
Spontaneous nucleotide exchange in low molecular weight GTPases by fluorescently labeled gamma-phosphate-linked GTP analogs.
Korlach J, Baird DW, Heikal AA, Gee KR, Hoffman GR, Webb WW. Proc Natl Acad Sci U S A. 2004 Mar 2; 101(9):2800-5. Epub 2004 Feb 18.
Biochemical characterization of the Ran-RanBP1-RanGAP system: are RanBP proteins and the acidic tail of RanGAP required for the Ran-RanGAP GTPase reaction? [Mol Cell Biol. 2003]
Biochemical characterization of the Ran-RanBP1-RanGAP system: are RanBP proteins and the acidic tail of RanGAP required for the Ran-RanGAP GTPase reaction?
Seewald MJ, Kraemer A, Farkasovsky M, Körner C, Wittinghofer A, Vetter IR. Mol Cell Biol. 2003 Nov; 23(22):8124-36.
Fluorescent coumarin-labeled nucleotides to measure ADP release from actomyosin. [Biophys J. 2001]
Fluorescent coumarin-labeled nucleotides to measure ADP release from actomyosin.
Webb MR, Corrie JE. Biophys J. 2001 Sep; 81(3):1562-9.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

Fluorescence approaches to the study of the p21ras GTPase mechanism.
Fluorescence approaches to the study of the p21ras GTPase mechanism.
Biochem Soc Trans. 1991 Apr ;19(2):432-7.

PubMed
Sacral and presacral tumors.
Sacral and presacral tumors.
Am J Surg. 1948 Dec ;76(6):687-93.

PubMed
Isolation and characterization of a cytochrome P450 of the IIA subfamily from hu...
Isolation and characterization of a cytochrome P450 of the IIA subfamily from human liver microsomes.
Eur J Biochem. 1991 Sep 1 ;200(2):511-7.

PubMed
A simple objective technique for measuring flexibility in thinking.
A simple objective technique for measuring flexibility in thinking.
J Gen Psychol. 1948 Jul ;39:15-22.

PubMed
Serum cholesterol concentration and coronary heart disease in population with lo...
Serum cholesterol concentration and coronary heart disease in population with low cholesterol concentrations.
BMJ. 1991 Aug 3 ;303(6797):276-82.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to: