Protein adsorption onto solid surfaces is a complex process playing an important role in biological systems, being crucial to maintain the three-dimensional conformation during molecules interaction. Polymers hybrid network could be used to perform immunoassays with the required high level specificity. We have developed a novel route for incorporating recombinant protein from Bovine Herpesvirus into a network using as template Poly(vinyl alcohol) chemically tailored with five organosilanes followed by FTIR spectroscopy characterization. Protein expressed in E. coli was incorporated into hybrid network; all the PVA-hybrids interacted with this biomolecule and FTIR vibrational bands have endorsed the results. PVA hybrids modified with 3-(triethoxysilyl) propylisocyanate-TESPI presented the best result in the immunoassay, enhancing the sera specificity in comparison with the commercial microplate. We have successfully developed organic-inorganic hybrid based on PVA-silane to be used in serological tests with recombinant protein as a promising tool for the development and production new diagnostic assays.