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Nat Biotechnol. 2008 Oct;26(10):1146-53. doi: 10.1038/nbt.1495.

The potential and challenges of nanopore sequencing.

Author information

  • 1Department of Molecular and Cell Biology, Harvard University, Cambridge, Massachusetts 02138, USA. dbranton@harvard.edu

Abstract

A nanopore-based device provides single-molecule detection and analytical capabilities that are achieved by electrophoretically driving molecules in solution through a nano-scale pore. The nanopore provides a highly confined space within which single nucleic acid polymers can be analyzed at high throughput by one of a variety of means, and the perfect processivity that can be enforced in a narrow pore ensures that the native order of the nucleobases in a polynucleotide is reflected in the sequence of signals that is detected. Kilobase length polymers (single-stranded genomic DNA or RNA) or small molecules (e.g., nucleosides) can be identified and characterized without amplification or labeling, a unique analytical capability that makes inexpensive, rapid DNA sequencing a possibility. Further research and development to overcome current challenges to nanopore identification of each successive nucleotide in a DNA strand offers the prospect of 'third generation' instruments that will sequence a diploid mammalian genome for approximately $1,000 in approximately 24 h.

PMID:
18846088
[PubMed - indexed for MEDLINE]
PMCID:
PMC2683588
Free PMC Article

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