Methods Mol Biol. 2009;493:103-23.

Helios Gene Gun-mediated transfection of the inner ear sensory epithelium.

Source

Laboratory of Molecular Genetics, Section on Human Genetics, NIDCD, National Institutes of Health, Rockville MD, USA.

Abstract

Helios Gene Gun-mediated transfection is a biolistic method for mechanical delivery of exogenous DNA into cells in vitro or in vivo. The technique is based on bombardment of a targeted cellular surface by micron- or submicron-sized DNA-coated gold particles that are accelerated by a pressure pulse of compressed helium gas. The main advantage of Helios Gene Gun-mediated transfections is that it functions well on various cell types, including terminally differentiated cells that are difficult to transfect, such as neurons or inner ear sensory hair cells, and cells in internal cellular layers, such as neurons in organotypic brain slices. The successful delivery of mRNA, siRNA, or DNA of practically any size can be achieved using biolistic transfection. This chapter provides a detailed description and critical evaluation of the methodology used to transfect cDNA expression constructs, including green fluorescent protein (GFP) tagged full-length cDNAs of myosin XVa, whirlin, and beta-actin, into cultured inner ear sensory epithelia using the Bio-Rad Helios Gene Gun.

PMID:: 18839344; [PubMed - indexed for MEDLINE]

LinkOut - more resources

Full Text Sources

Springer

Supplemental Content

Save items

Related citations in PubMed

Biolistic transfection of neuronal cultures using a hand-held gene gun. [Nat Protoc. 2006]
Biolistic transfection of neuronal cultures using a hand-held gene gun.
O'Brien JA, Lummis SC. Nat Protoc. 2006; 1(2):977-81.
Myosin XVa localizes to the tips of inner ear sensory cell stereocilia and is essential for staircase formation of the hair bundle. [Proc Natl Acad Sci U S A. 2003]
Myosin XVa localizes to the tips of inner ear sensory cell stereocilia and is essential for staircase formation of the hair bundle.
Belyantseva IA, Boger ET, Friedman TB. Proc Natl Acad Sci U S A. 2003 Nov 25; 100(24):13958-63. Epub 2003 Nov 10.
Biolistic transfection of neurons. [Sci STKE. 2000]
Biolistic transfection of neurons.
McAllister AK. Sci STKE. 2000 Sep 26; 2000(51):pl1. Epub 2000 Sep 26.
Review Plasmid-mediated gene transfer in neurons using the biolistics technique. [J Neurosci Methods. 1997]
Review Plasmid-mediated gene transfer in neurons using the biolistics technique.
Biewenga JE, Destrée OH, Schrama LH. J Neurosci Methods. 1997 Jan; 71(1):67-75.
Review Diolistics: incorporating fluorescent dyes into biological samples using a gene gun. [Trends Biotechnol. 2007]
Review Diolistics: incorporating fluorescent dyes into biological samples using a gene gun.
O'Brien JA, Lummis SC. Trends Biotechnol. 2007 Nov; 25(11):530-4. Epub 2007 Oct 22.

See reviews... See all...

Cited by 3 PubMed Central articles

Mechanotransduction in mouse inner ear hair cells requires transmembrane channel-like genes. [J Clin Invest. 2011]
Mechanotransduction in mouse inner ear hair cells requires transmembrane channel-like genes.
Kawashima Y, Géléoc GS, Kurima K, Labay V, Lelli A, Asai Y, Makishima T, Wu DK, Della Santina CC, Holt JR, et al. J Clin Invest. 2011 Dec; 121(12):4796-809. Epub 2011 Nov 21.
Twinfilin 2 regulates actin filament lengths in cochlear stereocilia. [J Neurosci. 2009]
Twinfilin 2 regulates actin filament lengths in cochlear stereocilia.
Peng AW, Belyantseva IA, Hsu PD, Friedman TB, Heller S. J Neurosci. 2009 Dec 2; 29(48):15083-8.
Review Lipid-based systemic delivery of siRNA. [Adv Drug Deliv Rev. 2009]
Review Lipid-based systemic delivery of siRNA.
Tseng YC, Mozumdar S, Huang L. Adv Drug Deliv Rev. 2009 Jul 25; 61(9):721-31. Epub 2009 Mar 26.

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

Helios Gene Gun-mediated transfection of the inner ear sensory epithelium.
Helios Gene Gun-mediated transfection of the inner ear sensory epithelium.
Methods Mol Biol. 2009 ;493:103-23.

PubMed
Influenza and pneumococcal vaccination rates among Vietnamese, Asian, and non-Hi...
Influenza and pneumococcal vaccination rates among Vietnamese, Asian, and non-Hispanic white Americans.
J Immigr Minor Health. 2010 Jun ;12(3):370-6. Epub 2008 Oct 7 .

PubMed
Effects of men and recipients' age on the reproductive outcome of an oocyte dona...
Effects of men and recipients' age on the reproductive outcome of an oocyte donation program.
J Assist Reprod Genet. 2008 Sep-Oct ;25(9-10):445-52. Epub 2008 Oct 7 .

PubMed
Phylogenetic evidence suggests that strains of Aeromonas hydrophila subsp. dhake...
Phylogenetic evidence suggests that strains of Aeromonas hydrophila subsp. dhakensis belong to the species Aeromonas aquariorum sp. nov.
Curr Microbiol. 2009 Jan ;58(1):76-80. Epub 2008 Oct 7 .

PubMed
Treatment of osteoblastoma at C7: a multidisciplinary approach. A case report an...
Treatment of osteoblastoma at C7: a multidisciplinary approach. A case report and review of the literature.
Eur Spine J. 2009 Jul ;18 Suppl 2:196-200. Epub 2008 Oct 7 .

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to:

Helios Gene Gun-mediated transfection of the inner ear sensory epithelium.

Source

Abstract

Publication Types, MeSH Terms, Substances

Publication Types

MeSH Terms

Substances

LinkOut - more resources

Full Text Sources

Supplemental Content

Save items

Related citations in PubMed

Cited by 3 PubMed Central articles

Related information

Recent activity

Simple NCBI Directory

Getting Started

Resources

Popular

Featured

NCBI Information