Format

Send to:

Choose Destination
See comment in PubMed Commons below
Comp Biochem Physiol B Biochem Mol Biol. 2009 Jan;152(1):38-46. doi: 10.1016/j.cbpb.2008.09.084. Epub 2008 Sep 19.

Isolation and characterization of the apolipoprotein multigene family in Hemibarbus mylodon (Teleostei: Cypriniformes).

Author information

  • 1Department of Aquaculture, Pukyong National University, Nam-gu, Busan 608-737, South Korea.

Abstract

We isolated and characterized the full-length cDNA sequences of nine apolipoproteins (apoA-I-1, apoA-I-2, apoA-IV, apoE-1, apoE-2, apoC-I, apoC-II, apo-14 kDa, and apoM) in the Korean spotted barbel Hemibarbus mylodon (Teleostei: Cypriniformes), an endangered fish species. Nucleotide sequences of all apolipoproteins of H. mylodon showed high levels of identity to those of cyprinid species. The secondary structures of their deduced amino acid sequences were generally conserved with previously reported vertebrate counterparts. Molecular phylogenetic analyses revealed that apolipoproteins from teleosts and avian/mammalian species were phylogenetically separated according to each encoded protein. Within each apolipoprotein lineage, teleosts consistently formed a strongly supported monophyletic group and were genetically separated from terrestrial vertebrates. Tissue distributions of apolipoprotein transcripts in H. mylodon were variable depending on apolipoprotein family members with relatively high expression levels in the liver and intestine. The overall spectrum of mRNA tissue distribution was wider in H. mylodon than in other teleosts and mammals. In addition, our findings showed the multiple isoforms of apoA-I and apoE were differentially modulated across tissues possibly with an isoform-specific role in a given tissue.

PMID:
18838126
[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Write to the Help Desk