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Fertil Steril. 2009 Jan;91(1):231-9. doi: 10.1016/j.fertnstert.2007.07.1330. Epub 2008 Sep 30.

Monocyte chemotactic protein-1 (MCP-1), its receptor, and macrophages in the perifollicular stroma during the human ovulatory process.

Author information

  • 1Department of Obstetrics and Gynecology, the Sahlgrenska Academy at Göteborg University, Göteborg, Sweden. pernilla.dahm-kahler@vgregion.se

Abstract

OBJECTIVE:

To evaluate the expression and localization of the macrophage-specific chemokine monocyte chemotactic protein-1 (MCP-1), CC chemokine receptor 2 (CCR2), and macrophages (CD68) in the perifollicular stroma of different phases of the human ovulatory process and its relation to macrophage influx.

DESIGN:

Experimental study on patient-controlled material.

SETTING:

University hospital.

PATIENT(S):

Twenty-eight women planned to undergo laparoscopic sterilization.

INTERVENTION(S):

Surgery was performed at either of four distinct ovulatory phases, and a biopsy sample was obtained from the perifollicular stroma of the ovulatory follicle.

MAIN OUTCOME MEASURE(S):

Real-time polymerase chain reaction, immunoblotting, and immunohistochemistry were used for measurements of MCP-1, CCR2, and macrophages.

RESULT(S):

The messenger RNA levels of MCP-1 in the perifollicular stroma increased from the preovulatory to the late ovulatory phase and declined during the postovulatory phase. A higher density of macrophages was found in the preovulatory and early ovulatory phases compared with late and postovulatory phases. Monocyte chemotactic protein-1 and CCR2 were present in the stroma. Protein expression of CD68 and CCR2 were identified in the four ovulatory phases.

CONCLUSION(S):

This study demonstrates an upregulation of MCP-1 in the stroma compartment around the human follicle during the ovulatory process, and a high density of macrophages was found at earlier phases. Thus, inflammation-like reactions are integral in the ovulatory process and may be targeted to stimulate or inhibit this process.

PMID:
18829011
[PubMed - indexed for MEDLINE]
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