Warning: The NCBI web site requires JavaScript to function. more...

My NCBI Sign In

Result Filters

Proc Natl Acad Sci U S A. 2008 Oct 7;105(40):15435-40. Epub 2008 Sep 29.

Caulobacter requires a dedicated mechanism to initiate chromosome segregation.

Toro E, Hong SH, McAdams HH, Shapiro L.

Source

Department of Developmental Biology, Stanford University School of Medicine, Beckman Center, B300, Stanford, CA 94305, USA.

Abstract

Chromosome segregation in bacteria is rapid and directed, but the mechanisms responsible for this movement are still unclear. We show that Caulobacter crescentus makes use of and requires a dedicated mechanism to initiate chromosome segregation. Caulobacter has a single circular chromosome whose origin of replication is positioned at one cell pole. Upon initiation of replication, an 8-kb region of the chromosome containing both the origin and parS moves rapidly to the opposite pole. This movement requires the highly conserved ParABS locus that is essential in Caulobacter. We use chromosomal inversions and in vivo time-lapse imaging to show that parS is the Caulobacter site of force exertion, independent of its position in the chromosome. When parS is moved farther from the origin, the cell waits for parS to be replicated before segregation can begin. Also, a mutation in the ATPase domain of ParA halts segregation without affecting replication initiation. Chromosome segregation in Caulobacter cannot occur unless a dedicated parS guiding mechanism initiates movement.

PMID:: 18824683; [PubMed - indexed for MEDLINE]
PMCID:: PMC2563096

Free PMC Article

Images from this publication.See all images (5) Free text

Fig. 1.

Fig. 3.

Fig. 5.

Fig. 2.

Fig. 4.

LinkOut - more resources

Full Text Sources

Other Literature Sources

Supplemental Content

Icon for HighWire Press

Icon for PubMed Central

Save items

loading

Related citations in PubMed

A moving DNA replication factory in Caulobacter crescentus. [EMBO J. 2001]
A moving DNA replication factory in Caulobacter crescentus.
Jensen RB, Wang SC, Shapiro L. EMBO J. 2001 Sep 3; 20(17):4952-63.
A polymeric protein anchors the chromosomal origin/ParB complex at a bacterial cell pole. [Cell. 2008]
A polymeric protein anchors the chromosomal origin/ParB complex at a bacterial cell pole.
Bowman GR, Comolli LR, Zhu J, Eckart M, Koenig M, Downing KH, Moerner WE, Earnest T, Shapiro L. Cell. 2008 Sep 19; 134(6):945-55.
The Caulobacter crescentus smc gene is required for cell cycle progression and chromosome segregation. [Proc Natl Acad Sci U S A. 1999]
The Caulobacter crescentus smc gene is required for cell cycle progression and chromosome segregation.
Jensen RB, Shapiro L. Proc Natl Acad Sci U S A. 1999 Sep 14; 96(19):10661-6.
Review Control of chromosome replication in caulobacter crescentus. [Annu Rev Microbiol. 2002]
Review Control of chromosome replication in caulobacter crescentus.
Marczynski GT, Shapiro L. Annu Rev Microbiol. 2002; 56:625-56. Epub 2002 Jan 30.
Review Cell cycle regulation in Caulobacter: location, location, location. [J Cell Sci. 2007]
Review Cell cycle regulation in Caulobacter: location, location, location.
Goley ED, Iniesta AA, Shapiro L. J Cell Sci. 2007 Oct 15; 120(Pt 20):3501-7.

See reviews... See all...

Cited by 19 PubMed Central articles

Filament depolymerization can explain chromosome pulling during bacterial mitosis. [PLoS Comput Biol. 2011]
Filament depolymerization can explain chromosome pulling during bacterial mitosis.
Banigan EJ, Gelbart MA, Gitai Z, Wingreen NS, Liu AJ. PLoS Comput Biol. 2011 Sep; 7(9):e1002145. Epub 2011 Sep 22.
High-throughput, subpixel precision analysis of bacterial morphogenesis and intracellular spatio-temporal dynamics. [Mol Microbiol. 2011]
High-throughput, subpixel precision analysis of bacterial morphogenesis and intracellular spatio-temporal dynamics.
Sliusarenko O, Heinritz J, Emonet T, Jacobs-Wagner C. Mol Microbiol. 2011 May; 80(3):612-27. Epub 2011 Mar 17.
Escherichia coli sister chromosome separation includes an abrupt global transition with concomitant release of late-splitting intersister snaps. [Proc Natl Acad Sci U S A. 2011]
Escherichia coli sister chromosome separation includes an abrupt global transition with concomitant release of late-splitting intersister snaps.
Joshi MC, Bourniquel A, Fisher J, Ho BT, Magnan D, Kleckner N, Bates D. Proc Natl Acad Sci U S A. 2011 Feb 15; 108(7):2765-70. Epub 2011 Jan 31.

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
References for this PMC Article
Citation referenced in PubMed article. Only valid for PubMed citations that are also in PMC.
Free in PMC
Free full text articles in PMC
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

Caulobacter requires a dedicated mechanism to initiate chromosome segregation.
Caulobacter requires a dedicated mechanism to initiate chromosome segregation.
Proc Natl Acad Sci U S A. 2008 Oct 7 ;105(40):15435-40. Epub 2008 Sep 29 .

PubMed
Depression and coronary heart disease: recommendations for screening, referral, ...
Depression and coronary heart disease: recommendations for screening, referral, and treatment: a science advisory from the American Heart Association Prevention Committee of the Council on Cardiovascular Nursing, Council on Clinical Cardiology, Council on Epidemiology and Prevention, and Interdisciplinary Council on Quality of Care and Outcomes Research: endorsed by the American Psychiatric Association.
Circulation. 2008 Oct 21 ;118(17):1768-75. Epub 2008 Sep 29 .

PubMed
Gay men who engage in substance use and sexual risk behaviour: a dual-risk group...
Gay men who engage in substance use and sexual risk behaviour: a dual-risk group with unique characteristics.
Int J STD AIDS. 2008 Oct ;19(10):698-703.

PubMed
Mind bomb 1 in the lymphopoietic niches is essential for T and marginal zone B c...
Mind bomb 1 in the lymphopoietic niches is essential for T and marginal zone B cell development.
J Exp Med. 2008 Oct 27 ;205(11):2525-36. Epub 2008 Sep 29 .

PubMed
The spindle assembly checkpoint is satisfied in the absence of interkinetochore ...
The spindle assembly checkpoint is satisfied in the absence of interkinetochore tension during mitosis with unreplicated genomes.
J Cell Biol. 2008 Oct 6 ;183(1):29-36. Epub 2008 Sep 29 .

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...

You are here: NCBI > Literature > PubMed

Write to the Help Desk