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Lab Chip. 2008 Oct;8(10):1617-20. doi: 10.1039/b807374k. Epub 2008 Aug 13.

Formation of artificial lipid bilayers using droplet dielectrophoresis.

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  • 1School of Electronics and Computer Science, University of Southampton, Southampton, UK.

Abstract

We describe the formation of artificial bilayer lipid membranes (BLMs) by the controlled, electrical manipulation of aqueous droplets immersed in a lipid-alkane solution. Droplet movement was generated using dielectrophoresis on planar microelectrodes covered in a thin insulator. Droplets, surrounded by lipid monolayers, were brought into contact and spontaneously formed a BLM. The method produced BLMs suitable for single-channel recording of membrane protein activity and the technique can be extended to create programmable BLM arrays and networks.

PMID:
18813381
[PubMed - indexed for MEDLINE]
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