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    Nat Struct Mol Biol. 2008 Oct;15(10):1119-21. Epub 2008 Sep 21.

    A torque component present in mitotic kinesin Eg5 revealed by three-dimensional tracking.

    Yajima J, Mizutani K, Nishizaka T.

    Source

    Department of Physics, Gakushuin University, 1-5-1 Mejiro, Toshima-ku, 171-8588 Tokyo, Japan.

    Abstract

    Mitotic kinesin Eg5 is a homotetrameric molecular motor that cross-links and slides microtubules. The extent to which Eg5 moves processively is not clear. Here we use three-dimensional tracking of a quantum dot attached to the microtubule in a motility assay to directly visualize the corkscrew motion of a sliding microtubule. We show that the rotational pitch of microtubule sliding conveniently reports on the processivity of the driving motors, confirming that two-headed Eg5 is much less processive than two-headed kinesin-1.

    PMID:
    18806799
    [PubMed - indexed for MEDLINE]

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