The cyclin-dependent kinase Cdk1 directly regulates vacuole inheritance

Yutian Peng; Lois S Weisman

doi:10.1016/j.devcel.2008.07.007

The cyclin-dependent kinase Cdk1 directly regulates vacuole inheritance

Dev Cell. 2008 Sep;15(3):478-485. doi: 10.1016/j.devcel.2008.07.007.

Authors

Yutian Peng¹, Lois S Weisman²

Affiliations

¹ Department of Cell & Developmental Biology, Life Sciences Institute, University of Michigan, Ann Arbor, MI 48109, USA; Department of Biochemistry, University of Iowa, Iowa City, IA 52242, USA.
² Department of Cell & Developmental Biology, Life Sciences Institute, University of Michigan, Ann Arbor, MI 48109, USA. Electronic address: lweisman@umich.edu.

Abstract

In budding yeast, vacuole inheritance is tightly coordinated with the cell cycle. The movement of vacuoles and several other organelles is actin-based and is mediated by interaction between the yeast myosin V motor Myo2 and organelle-specific adaptors. Myo2 binds to vacuoles via the adaptor protein Vac17, which binds to the vacuole membrane protein Vac8. Here we show that the yeast cyclin-dependent kinase Cdk1 phosphorylates Vac17 and that phosphorylation of Vac17 parallels cell cycle-dependent movement of the vacuole. Substitution of the Cdk1 sites in Vac17 decreases its interaction with Myo2 and causes a partial defect in vacuole inheritance. This defect is enhanced in the presence of Myo2 with mutated phosphorylation sites. Thus, Cdk1 appears to control the timing of vacuole movement. The presence of multiple predicted Cdk1 sites in other organelle-specific myosin V adaptors suggests that the inheritance of other cytoplasmic organelles may be regulated by a similar mechanism.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
CDC2 Protein Kinase / genetics
CDC2 Protein Kinase / metabolism*
Cell Cycle / physiology*
Lipoproteins / genetics
Lipoproteins / metabolism
Membrane Proteins / genetics
Membrane Proteins / metabolism
Molecular Sequence Data
Myosin Heavy Chains / genetics
Myosin Heavy Chains / metabolism
Myosin Type V / genetics
Myosin Type V / metabolism
Phosphorylation
Receptors, Cell Surface / genetics
Receptors, Cell Surface / metabolism*
Saccharomyces cerevisiae / cytology*
Saccharomyces cerevisiae / genetics
Saccharomyces cerevisiae / physiology
Saccharomyces cerevisiae Proteins / genetics
Saccharomyces cerevisiae Proteins / metabolism*
Vacuoles / metabolism*
Vesicular Transport Proteins / genetics
Vesicular Transport Proteins / metabolism*

Substances

Lipoproteins
MYO2 protein, S cerevisiae
Membrane Proteins
Receptors, Cell Surface
Saccharomyces cerevisiae Proteins
VAC17 protein, S cerevisiae
VAC8 protein, S cerevisiae
Vesicular Transport Proteins
CDC2 Protein Kinase
Myosin Type V
Myosin Heavy Chains

Abstract

Publication types

MeSH terms

Substances

Grants and funding