Genetics. 2008 Nov;180(3):1763-6. Epub 2008 Sep 14.

A simple polymerase chain reaction-based method for the construction of recombinase-mediated cassette exchange donor vectors.

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Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA.

Abstract

Here we describe a simple method for generating donor vectors suitable for targeted transgenesis via recombinase-mediated cassette exchange (RMCE) using the PhiC31 integrase. This PCR-based strategy employs small attB "tails" on the primers used to amplify a sequence of interest, permitting the rapid creation of transgenes for in vivo analysis.

PMID:: 18791223; [PubMed - indexed for MEDLINE]
PMCID:: PMC2581973

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A simple polymerase chain reaction-based method for the construction of recombinase-mediated cassette exchange donor vectors.

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