Display Settings:

Format

Send to:

Choose Destination
J Am Chem Soc. 2008 Oct 8;130(40):13186-7. doi: 10.1021/ja8037728. Epub 2008 Sep 13.

Conditional glycosylation in eukaryotic cells using a biocompatible chemical inducer of dimerization.

Author information

  • 1Department of Chemistry, University of California, Berkeley, California 94720, USA.

Abstract

Chemical inducers of dimerization (CIDs) are cell-permeable small molecules capable of dimerizing two protein targets. The most widely used CID, the natural product rapamycin and its relatives, is immunosuppressive due to interactions with endogenous targets and thus has limited utility in vivo. Here we report a new biocompatible CID, Tmp-SLF, which dimerizes E. coli DHFR and FKBP and has no endogenous mammalian targets that would lead to unwanted in vivo side effects. We employed Tmp-SLF to modulate gene expression in a yeast three-hybrid assay. Finally, we engineered the Golgi-resident glycosyltransferase FucT7 for tunable control by Tmp-SLF in mammalian cells.

PMID:
18788807
[PubMed - indexed for MEDLINE]
PMCID:
PMC2709988
Free PMC Article

Images from this publication.See all images (4)Free text

Figure 1
Figure 2
Figure 3
Figure 4
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for American Chemical Society Icon for PubMed Central
    Loading ...
    Write to the Help Desk