Display Settings:


Send to:

Choose Destination
See comment in PubMed Commons below
N Biotechnol. 2008 Oct-Dec;25(2-3):126-32. doi: 10.1016/j.nbt.2008.08.004. Epub 2008 Aug 20.

Cell-free protein synthesis: applications in proteomics and biotechnology.

Author information

  • The Babraham Institute, Babraham Research Campus, Cambridge CB22 3AT, UK. mingyue.he@bbsrc.ac.uk


Protein production is one of the key steps in biotechnology and functional proteomics. Expression of proteins in heterologous hosts (such as in E. coli) is generally lengthy and costly. Cell-free protein synthesis is thus emerging as an attractive alternative. In addition to the simplicity and speed for protein production, cell-free expression allows generation of functional proteins that are difficult to produce by in vivo systems. Recent exploitation of cell-free systems enables novel development of technologies for rapid discovery of proteins with desirable properties from very large libraries. This article reviews the recent development in cell-free systems and their application in the large scale protein analysis.

[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

How to join PubMed Commons

    Supplemental Content

    Loading ...
    Write to the Help Desk