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Biosci Biotechnol Biochem. 2008 Sep;72(9):2472-5. Epub 2008 Sep 7.

Restriction on conjugational transfer of pLS20 in Bacillus subtilis 168.

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  • 1Institute for Advanced Biosciences, Keio University, Tsuruoka, Japan.

Abstract

Conjugational transfer of pLS20 in Bacillus subtilis Marburg 168 is restricted by the BsuM restriction-modification system. Restriction efficiency was measured using pLS20 derivatives possessing various numbers of XhoI sites, which are known to be recognized by BsuM. An increase in XhoI sites clearly reduced the conjugational efficiency of pLS20 as compared with that of pUB110 plasmid lacking XhoI.

PMID:
18776676
[PubMed - indexed for MEDLINE]
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