Send to:

Choose Destination
See comment in PubMed Commons below
Nucleic Acids Symp Ser (Oxf). 2008;(52):345-6. doi: 10.1093/nass/nrn174.

Synthesis of four colors fluorescently labelled 3'-O-blocked nucleotides with fluoride cleavable blocking group and linker for array based Sequencing-by-Synthesis applications.

Author information

  • 1Institut für Organische Chemie und Chemische Biologie, J. W. Goethe Universität Frankfurt am Main, Max-von-Laue Str. 7, D-60438 Frankfurt am Main, Germany.


Reversible terminators having a fluoride cleavable 3'-O-blocking group are presented. Each nucleotide triphosphate is labelled by a fluorescent dye cleavable by the same reagent. We present here their synthesis, cleavage experiments and polymerase incorporation tests for a possible use in a process of Sequencing-by-Synthesis.

[PubMed - indexed for MEDLINE]
Free full text
PubMed Commons home

PubMed Commons

How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for HighWire
    Loading ...
    Write to the Help Desk