Curr Protoc Cytom. 2006 Aug;Chapter 13:Unit13.8.

Reagents and instruments for multiplexed analysis using microparticles.

Source

La Jolla Bioengineering Institute, La Jolla, California, USA.

Abstract

Multiplexed molecular analysis by means of flow cytometry using optically encoded microspheres is a rapidly expanding application that has its roots in the earliest days of flow cytometry. The approach is driven by increasing demand for analytical methods to measure large numbers of biomolecules quantitatively and sensitively in small volumes of sample. Encoded microspheres and flow cytometry have been employed for a wide range of multiplexed molecular analysis, and detailed protocols for many of these have been developed. The goal of this unit is to provide an overview of the concepts, instruments, and reagents that enable these assays.

PMID:: 18770838; [PubMed - indexed for MEDLINE]

MeSH Terms, Substances, Grant Support

MeSH Terms

Substances

Grant Support

R01 EB003824-02/EB/NIBIB NIH HHS/United States

LinkOut - more resources

Full Text Sources

John Wiley & Sons, Inc.

Supplemental Content

Save items

Related citations in PubMed

Review Multiplexed and microparticle-based analyses: quantitative tools for the large-scale analysis of biological systems. [Cytometry A. 2006]
Review Multiplexed and microparticle-based analyses: quantitative tools for the large-scale analysis of biological systems.
Nolan JP, Mandy F. Cytometry A. 2006 May; 69(5):318-25.
[Microbeads, nanobeads and cytometry: applications to the analysis and purification of cells and biomolecules]. [Pathol Biol (Paris). 2003]
[Microbeads, nanobeads and cytometry: applications to the analysis and purification of cells and biomolecules].
Lizard G, Duvillard L, Wedemeyer N, Muller C, Ghiringhelli F, Cesbron A, Poncelet P, Gallet F, Kahn E, Gambert P, et al. Pathol Biol (Paris). 2003 Sep; 51(7):418-27.
Suspension array technology: evolution of the flat-array paradigm. [Trends Biotechnol. 2002]
Suspension array technology: evolution of the flat-array paradigm.
Nolan JP, Sklar LA. Trends Biotechnol. 2002 Jan; 20(1):9-12.
Review Multiplexed molecular detection using encoded microparticles and nanoparticles. [Expert Rev Mol Diagn. 2006]
Review Multiplexed molecular detection using encoded microparticles and nanoparticles.
Li Y, Luo D. Expert Rev Mol Diagn. 2006 Jul; 6(4):567-74.
Multiplexed microsphere-based flow cytometric immunoassays. [Curr Protoc Cytom. 2006]
Multiplexed microsphere-based flow cytometric immunoassays.
Kellar KL, Mahmutovic AJ, Bandyopadhyay K. Curr Protoc Cytom. 2006 Feb; Chapter 13:Unit13.1.

See reviews... See all...

Recent activity

Clear Turn Off Turn On

Reagents and instruments for multiplexed analysis using microparticles.
Reagents and instruments for multiplexed analysis using microparticles.
Curr Protoc Cytom. 2006 Aug ;Chapter 13:Unit13.8.

PubMed
Preparation of autogenic human feeder cells for growth of human embryonic stem c...
Preparation of autogenic human feeder cells for growth of human embryonic stem cells.
Curr Protoc Stem Cell Biol. 2008 Mar ;Chapter 1:Unit 1C.5.1-1C.5.15.

PubMed
Cartilage integration: evaluation of the reasons for failure of integration duri...
Cartilage integration: evaluation of the reasons for failure of integration during cartilage repair. A review.
Eur Cell Mater. 2008 Sep 3 ;16:26-39.

PubMed
Hallucinogen use disorders among adult users of MDMA and other hallucinogens.
Hallucinogen use disorders among adult users of MDMA and other hallucinogens.
Am J Addict. 2008 Sep-Oct ;17(5):354-63.

PubMed
The dream of a philologist.
The dream of a philologist.
Acta Oncol. 2008 ;47(6):991-3.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to:

Reagents and instruments for multiplexed analysis using microparticles.

Source

Abstract

MeSH Terms, Substances, Grant Support

MeSH Terms

Substances

Grant Support

LinkOut - more resources

Full Text Sources

Supplemental Content

Save items

Related citations in PubMed

Recent activity

Simple NCBI Directory

Getting Started

Resources

Popular

Featured

NCBI Information