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Curr Protoc Microbiol. 2006 Sep;Chapter 15:Unit15J.1. doi: 10.1002/9780471729259.mc15j01s02.

Human immunodeficiency viruses: propagation, quantification, and storage.

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  • 1University of Massachusetts, Medical School, Worcester, Massachusetts, USA.


Described in this unit are basic protocols frequently used in the research of human immunodeficiency viruses (HIVs). Provided are methods for propagating and quantifying HIV, as well as recommendations for long-term storage. Background information about these methods is also provided and includes their advantages, disadvantages, and troubleshooting.

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