Abstract
Nucleic acids are substrates for different types of damage, but little is known about the fate of damaged RNAs. We addressed the existence of an RNA-damage response in yeast. The decay kinetics of GAL1p-driven mRNAs revealed a dose-dependent mRNA stabilization upon UV-irradiation that was not observed after heat or saline shocks, or during nitrogen starvation. UV-induced mRNA stabilization did not depend on DNA repair, damage checkpoint or mRNA degradation machineries. Notably, fluorescent in situ hybridization revealed that after UV-irradiation, polyadenylated mRNA accumulated in cytoplasmic foci that increased in size with time. In situ colocalization showed that these foci are not processing-bodies, eIF4E-, eIF4G-, and Pab1-containing bodies, stress granules, autophagy vesicles, or part of the secretory or endocytic pathways. These results point to the existence of a specific eukaryotic RNA-damage response, which leads to new polyadenylated mRNA-containing granules (UV-induced mRNA granules; UVGs). We propose that potentially damaged mRNAs, which may be deleterious to the cell, are temporarily stored in UVG granules to safeguard cell viability.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Autophagy / drug effects
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Autophagy / radiation effects
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Cytoplasmic Granules / drug effects
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Cytoplasmic Granules / metabolism*
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Cytoplasmic Granules / radiation effects*
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Dose-Response Relationship, Radiation
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Endocytosis / drug effects
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Endocytosis / radiation effects
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Galactokinase / genetics
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Galactokinase / metabolism
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Gene Expression Regulation, Fungal / drug effects
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Gene Expression Regulation, Fungal / radiation effects
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Heat-Shock Response / drug effects
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Heat-Shock Response / radiation effects
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Nitrogen / deficiency
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Poly A / metabolism
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Polyribosomes / drug effects
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Polyribosomes / metabolism
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Polyribosomes / radiation effects
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RNA Stability / drug effects
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RNA Stability / radiation effects
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Saccharomyces cerevisiae / cytology
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Saccharomyces cerevisiae / drug effects
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Saccharomyces cerevisiae / metabolism*
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Saccharomyces cerevisiae / radiation effects*
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Saccharomyces cerevisiae Proteins / genetics
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Saccharomyces cerevisiae Proteins / metabolism
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Sodium Chloride / pharmacology
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Ultraviolet Rays*
Substances
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RNA, Messenger
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Saccharomyces cerevisiae Proteins
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Poly A
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Sodium Chloride
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GAL1 protein, S cerevisiae
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Galactokinase
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Nitrogen