Format

Send to:

Choose Destination
See comment in PubMed Commons below
Langmuir. 2008 Oct 7;24(19):10786-90. doi: 10.1021/la703630a. Epub 2008 Aug 28.

Nanoneedle method for high-sensitivity low-background monitoring of protein activity.

Author information

  • 1Department of Physics, Division of Engineering, Brown University, Providence, Rhode Island 02912, USA.

Abstract

We describe a new method for measuring the activity of protein in miniscule quantities using a carbon nanotube nanoneedle. The unique features of this new method are (a) the immobilization of a few molecules; (b) subsequent translocation and isolation of them near the tip of a position-actuated nanoneedle; and (c) a fixed, defined, and unhindered molecular position to allow rapid real-time sensing and monitoring. The kinetic bioactivity of immobilized alkaline phosphatase (AP) molecules was measured as test model. Results show no decrease in enzymatic activity compared to that of the solution-phase enzyme reaction, suggesting that the immobilization provided unhindered access for ligand binding and minimal conformational modulation caused by undesired surface interactions.

PMID:
18759385
[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for American Chemical Society
    Loading ...
    Write to the Help Desk