Linkage disequilibrium (D) between SNPs at the 20q13 locus in the control cohort together with the corresponding Haploview gene track. The association signal resides in a region of LD that harbors several genes, including TNFRSF6B.

Colonic TNFRSF6B expression and serum DCR3 concentration. (a) Colon biopsies were obtained from healthy controls (n = 11, Crohn’s disease histological index of severity (CDHIS):0) and affected segments from individuals with Crohn’s disease with ileo-colonic (n = 18, mean ± s.e.m. CDHIS: 4.1 ± 0.7) or colon-only (n = 14, mean ± s.e.m CDHIS: 4.9 ± 1) location and individuals with UC (n = 10, mean ± s.e.m. CDHIS: 7.2 ± 0.6, P < 0.05 vs. CD groups). We prepared RNA and determined the global pattern of gene expression using the Affymetrix GeneChip Human Genome HG-U133 Plus 2.0 array. Data were normalized to an internal control reference sample, and then to the median value of the healthy control samples, to allow for comparison of mRNA expression in the IBD colon samples relative to the healthy control samples. Results for TNFRSF6B mRNA expression relative to controls are shown. (b) We determined the serum concentration of the TNFRSF6B gene product, DCR3, by ELISA in healthy controls (n = 10), individuals with IBD with the TNFRSF6B GGGG genotype (n = 17, carrying the major allelic variants: G at rs2315008 and G at rs4809330) and individuals with IBD with the TNFRSF6B TGAG genotype (n = 11, carrying the minor allelic variants: T at rs2315008 and A at rs4809330). *P < 0.05, **P < 0.01 vs. control, ***P < 0.05 vs. TNFRSF6B GGGG genotype.