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J Antimicrob Chemother. 2008 Nov;62(5):929-33. doi: 10.1093/jac/dkn334. Epub 2008 Aug 27.

Vibrio cholerae O1 from Accra, Ghana carrying a class 2 integron and the SXT element.

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  • 1Department of Microbiology, University of Ghana Medical School, PO Box 4236, Accra, Ghana.

Abstract

OBJECTIVES:

Vibrio cholerae O1 from a 2006 outbreak in Accra were commonly resistant to multiple antimicrobials and, in particular, to trimethoprim/sulfamethoxazole, drugs commonly used in the treatment of cholera. We sought to determine the genetic basis for trimethoprim/sulfamethoxazole resistance in outbreak isolates.

METHODS:

Twenty-seven isolates from the outbreak were screened by PCR and sequencing for class 1 and 2 integrons and for the SXT element.

RESULTS:

Twenty-one of the 27 isolates examined, all from the Accra metropolitan area, carried both SXT, an integrated chromosomal element, and a class 2 integron bearing dfrA1, sat and aadA1 cassettes. All these isolates had identical random amplification of polymorphic DNA profiles and two of them also carried a class 1 integron.

CONCLUSIONS:

Most strains characterized carried multiple elements conferring resistance to trimethoprim. This suggests that trimethoprim/sulfamethoxazole should not be used empirically in cholera treatment.

PMID:
18755696
[PubMed - indexed for MEDLINE]
PMCID:
PMC2566517
Free PMC Article
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