FEBS Lett. 2008 Sep 22;582(21-22):3230-6. Epub 2008 Aug 26.

Cholesterol displacement by ceramide in sphingomyelin-containing liquid-ordered domains, and generation of gel regions in giant lipidic vesicles.

Sot J, Ibarguren M, Busto JV, Montes LR, Goñi FM, Alonso A.

Source

Unidad de Biofísica (Centro Mixto CSIC-UPV/EHU), Universidad del País Vasco, P.O. Box 644, 48080 Bilbao, Spain.

Abstract

Fluorescence confocal microscopy and differential scanning calorimetry are used in combination to study the phase behaviour of bilayers composed of PC:PE:SM:Chol equimolecular mixtures, in the presence or absence of 10 mol% egg ceramide. In the absence of ceramide, separate liquid-ordered and liquid-disordered domains are observed in giant unilamellar vesicles. In the presence of ceramide, gel-like domains appear within the liquid-ordered regions. The melting properties of these gel-like domains resemble those of SM:ceramide binary mixtures, suggesting Chol displacement by ceramide from SM:Chol-rich liquid-ordered regions. Thus three kinds of domains coexist within a single vesicle in the presence of ceramide: gel, liquid-ordered, and liquid-disordered. In contrast, when 10 mol% egg diacylglycerol is added instead of ceramide, homogeneous vesicles, consisting only of liquid-disordered bilayers, are observed.

PMID:: 18755187; [PubMed - indexed for MEDLINE]

LinkOut - more resources

Full Text Sources

Medical

Cholesterol - MedlinePlus Health Information

Molecular Biology Databases

Supplemental Content

Related citations

Detergent-resistant, ceramide-enriched domains in sphingomyelin/ceramide bilayers. [Biophys J. 2006]
Detergent-resistant, ceramide-enriched domains in sphingomyelin/ceramide bilayers.
Sot J, Bagatolli LA, Goñi FM, Alonso A. Biophys J. 2006 Feb 1; 90(3):903-14. Epub 2005 Nov 11.
Absence of fluid-ordered/fluid-disordered phase coexistence in ceramide/POPC mixtures containing cholesterol. [Biophys J. 2006]
Absence of fluid-ordered/fluid-disordered phase coexistence in ceramide/POPC mixtures containing cholesterol.
Fidorra M, Duelund L, Leidy C, Simonsen AC, Bagatolli LA. Biophys J. 2006 Jun 15; 90(12):4437-51. Epub 2006 Mar 24.
The role of sphingomyelin in regulating phase coexistence in complex lipid model membranes: competition between ceramide and cholesterol. [Biochim Biophys Acta. 2008]
The role of sphingomyelin in regulating phase coexistence in complex lipid model membranes: competition between ceramide and cholesterol.
Staneva G, Chachaty C, Wolf C, Koumanov K, Quinn PJ. Biochim Biophys Acta. 2008 Dec; 1778(12):2727-39. Epub 2008 Aug 5.
Review Effects of ceramide and other simple sphingolipids on membrane lateral structure. [Biochim Biophys Acta. 2009]
Review Effects of ceramide and other simple sphingolipids on membrane lateral structure.
Goñi FM, Alonso A. Biochim Biophys Acta. 2009 Jan; 1788(1):169-77. Epub 2008 Sep 20.
Review Targeting membrane proteins to liquid-ordered phases: molecular self-organization explored by fluorescence correlation spectroscopy. [Chem Phys Lipids. 2006]
Review Targeting membrane proteins to liquid-ordered phases: molecular self-organization explored by fluorescence correlation spectroscopy.
Kahya N. Chem Phys Lipids. 2006 Jun; 141(1-2):158-68. Epub 2006 Apr 4.

See reviews... See all...

Cited by 6 PubMed Central articles

EGF receptor exposed to oxidative stress acquires abnormal phosphorylation and aberrant activated conformation that impairs canonical dimerization. [PLoS One. 2011]
EGF receptor exposed to oxidative stress acquires abnormal phosphorylation and aberrant activated conformation that impairs canonical dimerization.
Filosto S, Khan EM, Tognon E, Becker C, Ashfaq M, Ravid T, Goldkorn T. PLoS One. 2011; 6(8):e23240. Epub 2011 Aug 10.
Regulation of phosphatidic Acid metabolism by sphingolipids in the central nervous system. [J Lipids. 2011]
Regulation of phosphatidic Acid metabolism by sphingolipids in the central nervous system.
Pasquaré SJ, Gaveglio VL, Giusto NM. J Lipids. 2011; 2011:342576. Epub 2010 Nov 7.
Cholesterol displaces palmitoylceramide from its tight packing with palmitoylsphingomyelin in the absence of a liquid-disordered phase. [Biophys J. 2010]
Cholesterol displaces palmitoylceramide from its tight packing with palmitoylsphingomyelin in the absence of a liquid-disordered phase.
Busto JV, Sot J, Requejo-Isidro J, Goñi FM, Alonso A. Biophys J. 2010 Aug 9; 99(4):1119-28.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Compound (MeSH Keyword)
PubChem chemical compound records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

Cholesterol displacement by ceramide in sphingomyelin-containing liquid-ordered ...
Cholesterol displacement by ceramide in sphingomyelin-containing liquid-ordered domains, and generation of gel regions in giant lipidic vesicles.
FEBS Lett. 2008 Sep 22 ;582(21-22):3230-6. Epub 2008 Aug 26 .

PubMed
Size-dependent cellular uptake and expulsion of single-walled carbon nanotubes: ...
Size-dependent cellular uptake and expulsion of single-walled carbon nanotubes: single particle tracking and a generic uptake model for nanoparticles.
ACS Nano. 2009 Jan 27 ;3(1):149-58.

PubMed
Imaging clathrin dynamics in Drosophila melanogaster hemocytes reveals a role fo...
Imaging clathrin dynamics in Drosophila melanogaster hemocytes reveals a role for actin in vesicle fission.
Traffic. 2006 Dec ;7(12):1614-27. Epub 2006 Oct 2 .

PubMed
[Low prevalence of anti-centromere antibodies in scleroderma in Morocco (about 2...
[Low prevalence of anti-centromere antibodies in scleroderma in Morocco (about 272 cases)].
Ann Biol Clin (Paris). 2007 May-Jun ;65(3):291-7.

PubMed
Primary biliary cirrhosis accompanied by CREST syndrome.
Primary biliary cirrhosis accompanied by CREST syndrome.
South Med J. 2002 Sep ;95(9):1058-9.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Display Settings:

Send to:

Cholesterol displacement by ceramide in sphingomyelin-containing liquid-ordered domains, and generation of gel regions in giant lipidic vesicles.

Source

Abstract

Publication Types, MeSH Terms, Substances

Publication Types

MeSH Terms

Substances

LinkOut - more resources

Full Text Sources

Medical

Molecular Biology Databases

Supplemental Content

Related citations

Cited by 6 PubMed Central articles

Related information

Recent activity

Simple NCBI Directory

Getting Started

Resources

Popular

Featured

NCBI Information