Cytometry A. 2008 Oct;73(10):958-64.

A fast, fully automated cell segmentation algorithm for high-throughput and high-content screening.

Fenistein D, Lenseigne B, Christophe T, Brodin P, Genovesio A.

Source

Image Mining Group, Institut Pasteur Korea, Hawolgok-dong, Seongbuk-gu, Seoul 136-791, Korea. dfenistein@yahoo.com

Abstract

High-throughput, high-content screening (HT-HCS) of large compound libraries for drug discovery imposes new constraints on image analysis algorithms. Time and robustness are paramount while accuracy is intrinsically statistical. In this article, a fast and fully automated algorithm for cell segmentation is proposed. The algorithm is based on a strong attachment to the data that provide robustness and have been validated on the HT-HCS of large compound libraries and different biological assays. We present the algorithm and its performance, a description of its advantages and limitations, and a discussion of its range of application.

PMID:: 18752283; [PubMed - indexed for MEDLINE]

Free full text

Publication Types, MeSH Terms

Publication Types

Validation Studies

MeSH Terms

LinkOut - more resources

Full Text Sources

Other Literature Sources

Labome Researcher Resource - ExactAntigen/Labome

Supplemental Content

Save items

Related citations in PubMed

A novel tracing algorithm for high throughput imaging Screening of neuron-based assays. [J Neurosci Methods. 2007]
A novel tracing algorithm for high throughput imaging Screening of neuron-based assays.
Zhang Y, Zhou X, Degterev A, Lipinski M, Adjeroh D, Yuan J, Wong ST. J Neurosci Methods. 2007 Feb 15; 160(1):149-62. Epub 2006 Sep 20.
DetecTiff: a novel image analysis routine for high-content screening microscopy. [J Biomol Screen. 2009]
DetecTiff: a novel image analysis routine for high-content screening microscopy.
Gilbert DF, Meinhof T, Pepperkok R, Runz H. J Biomol Screen. 2009 Sep; 14(8):944-55. Epub 2009 Jul 29.
Segmentation of clustered nuclei with shape markers and marking function. [IEEE Trans Biomed Eng. 2009]
Segmentation of clustered nuclei with shape markers and marking function.
Cheng J, Rajapakse JC. IEEE Trans Biomed Eng. 2009 Mar; 56(3):741-8. Epub 2008 Nov 11.
Review Requirements, features, and performance of high content screening platforms. [Methods Mol Biol. 2007]
Review Requirements, features, and performance of high content screening platforms.
Gough AH, Johnston PA. Methods Mol Biol. 2007; 356:41-61.
Review Computer vision for microscopy diagnosis of malaria. [Malar J. 2009]
Review Computer vision for microscopy diagnosis of malaria.
Tek FB, Dempster AG, Kale I. Malar J. 2009 Jul 13; 8:153. Epub 2009 Jul 13.

See reviews... See all...

Cited by 4 PubMed Central articles

High content phenotypic cell-based visual screen identifies Mycobacterium tuberculosis acyltrehalose-containing glycolipids involved in phagosome remodeling. [PLoS Pathog. 2010]
High content phenotypic cell-based visual screen identifies Mycobacterium tuberculosis acyltrehalose-containing glycolipids involved in phagosome remodeling.
Brodin P, Poquet Y, Levillain F, Peguillet I, Larrouy-Maumus G, Gilleron M, Ewann F, Christophe T, Fenistein D, Jang J, et al. PLoS Pathog. 2010 Sep 9; 6(9):e1001100. Epub 2010 Sep 9.
High content screening identifies decaprenyl-phosphoribose 2' epimerase as a target for intracellular antimycobacterial inhibitors. [PLoS Pathog. 2009]
High content screening identifies decaprenyl-phosphoribose 2' epimerase as a target for intracellular antimycobacterial inhibitors.
Christophe T, Jackson M, Jeon HK, Fenistein D, Contreras-Dominguez M, Kim J, Genovesio A, Carralot JP, Ewann F, Kim EH, et al. PLoS Pathog. 2009 Oct; 5(10):e1000645. Epub 2009 Oct 30.
Automatic analysis of dividing cells in live cell movies to detect mitotic delays and correlate phenotypes in time. [Genome Res. 2009]
Automatic analysis of dividing cells in live cell movies to detect mitotic delays and correlate phenotypes in time.
Harder N, Mora-Bermúdez F, Godinez WJ, Wünsche A, Eils R, Ellenberg J, Rohr K. Genome Res. 2009 Nov; 19(11):2113-24. Epub 2009 Oct 1.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

A fast, fully automated cell segmentation algorithm for high-throughput and high...
A fast, fully automated cell segmentation algorithm for high-throughput and high-content screening.
Cytometry A. 2008 Oct ;73(10):958-64.

PubMed
Range of motion in contemporary total hip arthroplasty. The impact of modular he...
Range of motion in contemporary total hip arthroplasty. The impact of modular head-neck components.
J Arthroplasty. 1991 Jun ;6(2):97-101.

PubMed
Physical activity in the prevention and treatment of anxiety and depression.
Physical activity in the prevention and treatment of anxiety and depression.
Nord J Psychiatry. 2008 ;62 Suppl 47:25-9.

PubMed
Ceramic head implantation failures.
Ceramic head implantation failures.
J Arthroplasty. 1991 Jun ;6(2):185-8.

PubMed
Efficacy of intraoperative autotransfusion in primary total hip arthroplasty.
Efficacy of intraoperative autotransfusion in primary total hip arthroplasty.
J Arthroplasty. 1991 Jun ;6(2):157-61.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to: