J Chromatogr. 1991 Apr 19;565(1-2):441-6.

Rapid high-performance liquid chromatographic assay for total homocysteine levels in human serum.

Ubbink JB, Hayward Vermaak WJ, Bissbort S.

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Department of Chemical Pathology, Faculty of Medicine, University of Pretoria, South Africa.

Abstract

A rapid, isocratic high-performance liquid chromatographic (HPLC) method is described for the determination of total homocysteine levels in human serum. Prior to reversed-phase HPLC analysis, the serum thiols were derivatized with SBD-F (ammonium 7-fluorobenzo-2-oxa-1,3-diazole-4-sulphonate), a thiolspecific fluorogenic probe which is commercially available. Retention of SBD-homocysteine was sensitive to pH, and a mobile phase pH of 2.1 ensured baseline separation of serum thiols within 6 min. The method is simple, sensitive, reproducible (between-run coefficient of variation of 6.6%) and very suitable for routine determination of serum homocysteine levels in a clinical pathology laboratory.

PMID:: 1874890; [PubMed - indexed for MEDLINE]

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Research Support, Non-U.S. Gov't

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Homocysteine

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Rapid high-performance liquid chromatographic assay for total homocysteine levels in human serum.

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Cited by 38 PubMed Central articles

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