Display Settings:

Format

Send to:

Choose Destination
See comment in PubMed Commons below
Phys Med Biol. 2008 Sep 21;53(18):5077-91. doi: 10.1088/0031-9155/53/18/015. Epub 2008 Aug 22.

Changes in wall motion and blood flow in the outflow tract of chick embryonic hearts observed with optical coherence tomography after outflow tract banding and vitelline-vein ligation.

Author information

  • 1Division of Biomedical Engineering, Oregon Health & Science University, 3303 SW Bond Ave., CH13B, Portland, OR 97239, USA.

Abstract

Optical coherence tomography (OCT) is a non-contact, non-invasive and high-resolution imaging technique, suited to study early cardiovascular development. Alterations in hemodynamic conditions during early development are known to lead to cardiac defects, presumably as a result of changes in cardiac biomechanics produced by the alterations. In this paper, we demonstrate the use of a spectral domain OCT in visualizing and quantifying changes in cardiac wall motion and blood-flow velocities under normal and altered hemodynamic conditions in chicken embryos at an early stage of development (Hamburger-Hamilton stage HH18, approximately 3 days of incubation), focusing on the heart outflow tract (OFT). The OCT system employed acquired simultaneously microstructural and blood-flow images at a rate of 92 frames s(-1)with a spatial resolution of approximately 10 microm. OCT imaging allowed in vivo visualization of the OFT microstructures, e.g. the lumen, cardiac cushions and myocardium. We found that alterations in hemodynamic conditions, through OFT banding and vitelline-vein ligation, changed blood-flow velocities through the OFT, as expected. Further, OCT allowed quantification of changes in the dynamics of OFT wall motion. Our results therefore establish the utility of spectral domain OCT to study the influence of hemodynamic conditions on heart development in intact, in vivo chicken embryo models.

PMID:
18723935
[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Icon for IOP Publishing Ltd.
    Loading ...
    Write to the Help Desk