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Opt Express. 2008 Aug 18;16(17):13315-22.

Nonlinear immunofluorescent assay for androgenic hormones based on resonant structures.

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  • 1ICFO-Institut de Ciencies Fotoniques, Ada Canal Olimpic s/n, 08600 Castelldefels, Barcelona, Spain.


We report for the first time the use of two photon fluorescence as detection method of affinity binding reactions. We use a resonant grating waveguide structure as platform enhancement for detecting the interaction between fluorescent labeled Boldenone, a non-natural androgenic hormone, and a specific anti-anabolic antibody. We were able to detect a surface coverage of approximately 0.7 ng/mm(2).

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