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Opt Express. 2008 Aug 18;16(17):12746-56.

In vivo measurement of erythrocyte velocity and retinal blood flow using adaptive optics scanning laser ophthalmoscopy.

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  • 1School of Optometry, Indiana University, 800 E Atwater Avenue, Bloomington, Indiana 47405, USA.


In vivo measurement of retinal blood flow is obtained by measuring the blood velocity of erythrocytes and lumen diameters of the blood vessels using an adaptive optics scanning laser ophthalmoscope. Erythrocyte velocity is measured by tracking erythrocytes moving across a horizontal scanning line. This approach provides high temporal bandwidth measurements, allowing the fluctuation of blood flow during cardiac cycles to be measured. The technique is most applicable to medium-sized blood vessels.

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