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Opt Express. 2008 Aug 18;16(17):12640-9.

Two-photon fluorescence correlation spectroscopy through a dual-clad optical fiber.

Author information

  • 1Center for Ultrafast Optical Science, University of Michigan, 2200 Bonisteel Blvd., Ann Arbor, MI 48109-2099, USA. ycchang@umich.edu

Abstract

We report on the utilization of a dual-clad optical fiber for two-photon excited fluorescence correlation spectroscopy (FCS). High excitation efficiency is obtained by sending prechirped ultrafast pulses through the single-mode core of the fiber, while the fluorescence collection efficiency is enhanced because of the larger numerical aperture of the inner cladding. We show that the utilization of a dual-clad fiber is critical for ultrasensitive two-photon fluorescence detection. Our system has the ability to detect fluorescent nanospheres as small as 12 nm in radius. Quantum dots of radius 7 nm are also measured and show excellent signal to noise ratio. The particle sizes obtained from the fiber FCS system were confirmed by measurements using a commercial dynamic light scattering (DLS) system.

PMID:
18711501
[PubMed - indexed for MEDLINE]
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