Trends Cell Biol. 2008 Sep;18(9):443-50. Epub 2008 Aug 14.

Chromophore-assisted laser inactivation in cell biology.

Jacobson K, Rajfur Z, Vitriol E, Hahn K.

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Department of Cell and Developmental Biology, University of North Carolina-Chapel Hill, Chapel Hill, NC 27599-7090, USA. frap@med.unc.edu

Abstract

Chromophore-assisted laser inactivation (CALI) is a technique whereby engineered proteins and dye molecules that produce substantial amounts of reactive oxygen species upon absorption of light are used to perturb biological systems in a spatially and temporally defined manner. CALI is an important complement to conventional genetic and pharmacological manipulations. In this review, we examine the applications of CALI to cell biology and discuss the underlying photochemical mechanisms that mediate this powerful technique.

PMID:: 18706812; [PubMed - indexed for MEDLINE]

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