Display Settings:

Format

Send to:

Choose Destination
See comment in PubMed Commons below
Mol Cell Proteomics. 2009 Jan;8(1):32-44. doi: 10.1074/mcp.M800139-MCP200. Epub 2008 Aug 12.

Quantitative proteomics reveals GIMAP family proteins 1 and 4 to be differentially regulated during human T helper cell differentiation.

Author information

  • 1Turku Centre for Biotechnology, University of Turku and Abo Akademi University, Tykistökatu 6B, FI-20520 Turku, Finland.

Abstract

T helper (Th) cells differentiate into functionally distinct effector cell subsets of which Th1 and Th2 cells are best characterized. Besides T cell receptor signaling, IL-12-induced STAT4 and T-bet- and IL-4-induced STAT6 and GATA3 signaling pathways are the major players regulating the Th1 and Th2 differentiation process, respectively. However, there are likely to be other yet unknown factors or pathways involved. In this study we used quantitative proteomics exploiting cleavable ICAT labeling and LC-MS/MS to identify IL-4-regulated proteins from the microsomal fractions of CD4(+) cells extracted from umbilical cord blood. We were able to identify 557 proteins of which 304 were also quantified. This study resulted in the identification of the down-regulation of small GTPases GIMAP1 and GIMAP4 by IL-4 during Th2 differentiation. We also showed that both GIMAP1 and GIMAP4 genes are up-regulated by IL-12 and other Th1 differentiation-inducing cytokines in cells induced to differentiate toward Th1 lineage and down-regulated by IL-4 in cells induced to Th2. Our results indicate that the GIMAP (GTPase of the immunity-associated protein) family of proteins is differentially regulated during Th cell differentiation.

PMID:
18701445
[PubMed - indexed for MEDLINE]
PMCID:
PMC2621005
Free PMC Article

Images from this publication.See all images (8)Free text

F ig . 1.
F ig . 2.
F ig . 3.
F ig . 4.
F ig . 5.
F ig . 6.
F ig . 7.
F ig . 8.
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for HighWire Icon for PubMed Central
    Loading ...
    Write to the Help Desk