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Mol Cell. 2008 Aug 8;31(3):383-94. doi: 10.1016/j.molcel.2008.06.018.

General and versatile autoinhibition of PLC isozymes.

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  • 1Department of Pharmacology, The University of North Carolina School of Medicine, Chapel Hill, NC 27599, USA.

Abstract

Phospholipase C (PLC) isozymes are directly activated by heterotrimeric G proteins and Ras-like GTPases to hydrolyze phosphatidylinositol 4,5-bisphosphate into the second messengers diacylglycerol and inositol 1,4,5-trisphosphate. Although PLCs play central roles in myriad signaling cascades, the molecular details of their activation remain poorly understood. As described here, the crystal structure of PLC-beta2 illustrates occlusion of the active site by a loop separating the two halves of the catalytic TIM barrel. Removal of this insertion constitutively activates PLC-beta2 without ablating its capacity to be further stimulated by classical G protein modulators. Similar regulation occurs in other PLC members, and a general mechanism of interfacial activation at membranes is presented that provides a unifying framework for PLC activation by diverse stimuli.

PMID:
18691970
[PubMed - indexed for MEDLINE]
PMCID:
PMC2702322
Free PMC Article
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