Rev Sci Instrum. 2008 Jul;79(7):076101.

Label-free, all-electrical, in situ human epidermal growth receptor 2 detection.

Capobianco JA, Shih WY, Yuan QA, Adams GP, Shih WH.

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Department of Materials Science and Engineering, Drexel University, Philadelphia, Pennsylvania 19104, USA.

Abstract

Using 3-mercaptopropyltrimethoxysilane (MPS)-coated (PbMg 1/3 Nb 2/3 O3)0.63-(PbTiO3)0.37 (PMN-PT)/tin and lead zirconate titanate/glass piezoelectric microcantilever sensors (PEMSs) with single-chain variable fragment (scFv) immobilized on the MPS surface, we have demonstrated real-time, label-free detection of human epidermal growth factor receptor 2 (Her2) in a background of 1 mg/ml bovine serum albumin. Coupled with a scFv with a KD of 3.4 x 10(-8)M, the MPS-insulated PMN-PT/tin PEMS 560 microm long and 720 microm wide exhibited a Her2 concentration sensitivity of 5 ng/ml in a background of 1 mg/ml BSA.

PMID:: 18681738; [PubMed - indexed for MEDLINE]
PMCID: PMC2678786

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Label-free, all-electrical, in situ human epidermal growth receptor 2 detection.

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