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Mol Cell Biol. 2008 Oct;28(19):6022-32. doi: 10.1128/MCB.00684-08. Epub 2008 Aug 4.

The T body, a new cytoplasmic RNA granule in Saccharomyces cerevisiae.

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  • 1Center for mRNP Biogenesis and Metabolism, Department of Molecular Biology, University of Aarhus, C.F. Møllers Allé, Building 1130, 8000 Arhus C, Denmark.

Abstract

A large share of mRNA processing and packaging events occurs cotranscriptionally. To explore the hypothesis that transcription defects may affect mRNA fate, we analyzed poly(A)(+) RNA distribution in Saccharomyces cerevisiae strains harboring mutations in Rpb1p, the largest subunit of RNA polymerase II. In certain rpb1 mutants, a poly(A)(+) RNA granule, distinct from any known structure, strongly accumulated in a confined space of the cytoplasm. RNA and protein expressed from Ty1 retrovirus-like elements colocalized with this new granule, which we have consequently named the T body. A visual screen revealed that the deletion of most genes with proposed functions in Ty1 biology unexpectedly does not alter T-body levels. In contrast, the deletion of genes encoding the Mediator transcription initiation factor subunits Srb2p and Srb5p as well as the Ty1 transcriptional regulator Spt21p greatly enhances T-body formation. Our data disclose a new cellular body putatively involved in the assembly of Ty1 particles and suggest that the cytoplasmic fate of mRNA can be affected by transcription initiation events.

PMID:
18678648
[PubMed - indexed for MEDLINE]
PMCID:
PMC2546999
Free PMC Article
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