Nat Methods. 2008 Oct;5(10):865-7. Epub 2008 Aug 1.

Caenorhabditis elegans mutant allele identification by whole-genome sequencing.

Sarin S, Prabhu S, O'Meara MM, Pe'er I, Hobert O.

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Department of Biochemistry and Molecular Biophysics, Howard Hughes Medical Institute, Columbia University Medical Center, New York, New York 10032, USA.

Abstract

Identification of the molecular lesion in Caenorhabditis elegans mutants isolated through forward genetic screens usually involves time-consuming genetic mapping. We used Illumina deep sequencing technology to sequence a complete, mutant C. elegans genome and thus pinpointed a single-nucleotide mutation in the genome that affects a neuronal cell fate decision. This constitutes a proof-of-principle for using whole-genome sequencing to analyze C. elegans mutants.

PMID:: 18677319; [PubMed - indexed for MEDLINE]
PMCID: PMC2574580

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