Send to:

Choose Destination
See comment in PubMed Commons below
Appl Environ Microbiol. 2008 Sep;74(18):5769-75. doi: 10.1128/AEM.00468-08. Epub 2008 Aug 1.

Engineering of an Escherichia coli strain for the production of 3-methyl-1-butanol.

Author information

  • 1University of California, Department of Chemical and Biomolecular Engineering, 5531 Boelter Hall, Los Angeles, CA 90095, USAdu.


3-Methyl-1-butanol is a potential fuel additive or substitute. Previously this compound was identified in small quantities in yeast fermentation as one of the fusel alcohols. In this work, we engineered an Escherichia coli strain to produce 3-methyl-1-butanol from glucose via the host's amino acid biosynthetic pathways. Strain improvement with the removal of feedback inhibition and competing pathways increased the selectivity and productivity of 3-methyl-1-butanol. This work demonstrates the feasibility of production of 3-methyl-1-butanol as a biofuel and shows promise in using E. coli as a host for production.

[PubMed - indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for HighWire Icon for PubMed Central
    Loading ...
    Write to the Help Desk