Display Settings:

Format

Send to:

Choose Destination
Fungal Genet Biol. 2008 Oct;45(10):1430-8. doi: 10.1016/j.fgb.2008.07.004. Epub 2008 Jul 15.

Cytoplasmic localization of sterol transcription factors Upc2p and Ecm22p in S. cerevisiae.

Author information

  • 1Department of Global Health, School of Medicine and School of Public Health and Community Medicine, University of Washington, USA.

Abstract

Ergosterol homeostasis is a critical process for fungal cells. Paralogous zinc cluster transcription factors Upc2p and Ecm22p are major regulators of ergosterol biosynthesis in Saccharomyces cerevisiae. Upc2p and Ecm22p sense and respond to sterol depletion but their mechanism of activation has not been defined. Subcellular localization and functional expression of Upc2p-GFP and Ecm22p-GFP was monitored by fluorescence microscopy and flow cytometry in live yeast cells. Both fusion proteins localized to intracellular membranes and to perinuclear foci. Perinuclear localization of Upc2p-GFP and Ecm22p-GFP was increased when ergosterol biosynthesis was blocked by azole drug treatment. Nuclear localization in response to sterol depletion is consistent with the hypothesis that Upc2p and Ecm22p are trafficked from a membrane to the nucleus as a post-translational mechanism of sterol sensing.

PMID:
18675371
[PubMed - indexed for MEDLINE]
PMCID:
PMC2580778
Free PMC Article

Images from this publication.See all images (4)Free text

Figure 1
Figure 2
Figure 3
Figure 4
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science Icon for PubMed Central
    Loading ...
    Write to the Help Desk