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    Nature. 2008 Jul 31;454(7204):E3-4; discussion E4-5.

    Does Rft1 flip an N-glycan lipid precursor?

    Frank CG, Sanyal S, Rush JS, Waechter CJ, Menon AK.

    Department of Biochemistry, Weill Cornell Medical College, New York, New York 10065, USA.

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    Protein N-glycosylation requires flipping of the glycolipid Man(5)GlcNAc(2)-diphosphate dolichol (Man(5)GlcNAc(2)-PP-Dol) across the endoplasmic reticulum (ER). Helenius et al. report genetic evidence suggesting that Rft1, an essential ER membrane protein in yeast, is required directly to translocate Man(5)GlcNAc(2)-PP-Dol. We now show that a specific ER protein(s), but not Rft1, is required to flip Man(5)GlcNAc(2)-PP-Dol in reconstituted vesicles. Rft1 may have a critical accessory role in translocating Man(5)GlcNAc(2)-PP-Dol in vivo, but the Man(5)GlcNAc(2)-PP-Dol flippase itself remains to be identified.

    PMID: 18668045 [PubMed - indexed for MEDLINE]

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