Illustration of HSV-1 gD showing the relative positions of T-cell epitope peptides used in this study. HSV-1 (strain 17) gD regions carrying potential human class II-restricted T-cell epitopes predicted by the TEPITOPE computer-assisted algorithm based on known HLA-peptide-TCR interactions are shown. The amino acid sequence, in single-letter code, and the peptide positions based on the full-length sequence of gD are shown. The black box represents the transmembrane domain.

CD4⁺ T-cell proliferative response to HSV-1 gD-derived epitopes. (A) Fresh peripheral blood-derived CD4⁺ T cells were isolated and stimulated in vitro with 12 individual gD peptides in a 6-day proliferation assay as described in Materials and Methods. The Tp-cell responses of Caucasian (white) and non-Caucasian (nonwhite) ethnic groups against each peptide, assessed by a [³H]thymidine incorporation assay, were compared. Proliferative responses were expressed as Δcpm values (counts per minute of cells incubated with peptide − counts per minute of cells without peptide). The mean of each group is shown as a solid horizontal line. An analysis of T-cell responses in 22 seronegative individuals is shown. A positive T-cell proliferative response was defined on the basis of the responses of HSV-seronegative control individuals, with a Δcpm value of ≥1,000 and an SI (counts per minute of cells incubated with peptide/counts per minute of cells without peptide) of ≥2. The values at the bottom of each data set (i.e., 30/35) are the mean number of responders/total number of individuals tested. The value at the top of each data set (i.e., 11.5) is the mean SI of each of the two groups, i.e., white or nonwhite. The P values compare the Tp-cell responses between white and nonwhite groups by one-way ANOVA. An asterisk in parentheses indicates a peptide that induced higher T-cell proliferation compared with HSV-seronegative control individuals (i.e., significantly higher SI and Δcpm value). (B) Analysis of HLA-DR restriction and functional frequencies of HSV-1 gD peptide-specific CD4⁺ T cells. Blocking of PBMC proliferation in response to the gD_1-29 and gD_49-82 peptides by specific anti-HLA-DR MAb.

Gender differences in Tp-cell responses to HSV-1 gD epitope peptides detected in healthy, asymptomatic, HSV-seropositive individuals. (A and D) Peptide-specific Tp-cell responses in HSV-1- and/or HSV-2-seropositive, asymptomatic, healthy individuals were examined as described in the legend to Fig. 2. The P values in panel A indicate statistically significant differences in Tp-cell responses between men and women (P < 0.05, ANOVA test). Gender dependence of IFN-γ-producing CD4⁺ T cells specific to 12 gD peptides (B) and to an irrelevant CD4⁺ T-cell epitope peptide from OVA (OVA_323-339) (C) was detected by ELISPOT in HSV-seropositive, asymptomatic individuals. PBMC derived from HSV-1/HSV-2-seropositive, asymptomatic men and women were stimulated with 12 gD peptides for 5 days, and IFN-γ ELISPOT assays were performed as described in Materials and Methods.

Gender difference in gD epitope-specific CD4⁺ T-cell responses detected in HLA-DR1 B1*0101 and HLA-DR4 B1*0401 Tg mice. Age-matched HLA-DR1 B1*0101 (HLA-DR1) and HLA-DR4 B1*0401 (HLA-DR4) Tg mice were infected ocularly with HSV-1 (McKrae) (A) or immunized intraperitoneally with VVgD (B). Spleens were harvest on day 14 postinfection and stimulated with each of the 12 individual peptides for 5 days, and peptide-specific IFN-γ-producing CD4⁺ T cells were detected as described in Materials and Methods. (C) CD4⁺ T-cell lines specific to gender-dependent gD_49-82, gD_77-104, and gD_121-152 were established from HSV-1-infected HLA Tg mice and restimulated in vitro with either the corresponding specific gD peptide or a heterologous HLA-DR-restricted T-cell epitope from HSV-1 gB (i.e., gB_161-175). The numbers of SFC producing IFN-γ are shown for each peptide as mean values ± standard deviations from two independent experiments. The P values are for comparisons of the IFN-γ-producing CD4⁺ T-cell responses of age-matched females and males by one-way ANOVA.