Display Settings:

Format

Send to:

Choose Destination
See comment in PubMed Commons below
Fungal Genet Biol. 2008 Oct;45(10):1422-9. doi: 10.1016/j.fgb.2008.06.009. Epub 2008 Jul 11.

Requirement of LaeA for secondary metabolism and sclerotial production in Aspergillus flavus.

Author information

  • 1Department of Biology, Xavier University of Louisiana, New Orleans, LA 70125, USA. skale@xula.edu

Abstract

The nuclear regulator LaeA has been shown to govern production of multiple secondary metabolites in Aspergillus nidulans and Aspergillus fumigatus. Herein we examine the role of this protein in Aspergillus flavus. Similarly as in other Aspergilli, LaeA had a major effect on A. flavus secondary metabolism where DeltalaeA and over-expression laeA (OE::laeA) strains yielded opposite phenotypes resulting in decreased (increased) secondary metabolite production. The two mutant strains also exhibited striking morphological phenotypes in the loss (increase) of sclerotial production in comparison to wildtype. Growth on seed was marked by decreased (increased) conidial and aflatoxin production of the respective mutants; this was accompanied by decreased lipase activity in DeltalaeA, an enzymatic process correlated with seed maceration. Transcriptional examination of the mutants showed LaeA negatively regulates expression of its recently identified nuclear partner VeA, another global regulator of A. flavus secondary metabolites and sclerotia.

PMID:
18667168
[PubMed - indexed for MEDLINE]
PMCID:
PMC2845523
Free PMC Article

Images from this publication.See all images (5)Free text

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science Icon for PubMed Central
    Loading ...
    Write to the Help Desk